Construct: shRNA TRCN0000279030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009321.2-236s21c1
DNA Barcode:
GATCTTCAGCAGATATTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbca (21371)
Gene Description:
tubulin cofactor A
Transcript:
RefSeq NM_009321.2 (CURRENT)
Match location:
Position 236 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTTCAGCAGATATTAGAA
Hairpin Sequence:
5'-CCGG-GATCTTCAGCAGATATTAGAA-CTCGAG-TTCTAATATCTGCTGAAGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTTCAGCAGATATTAGAACTCGAGTTCTAATATCTGCTGAAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTTCAGCAGATATTAGAACTCGAGTTCTAATATCTGCTGAAGATC-3'

Other clones with same target sequence:

  1. TRCN0000177110