Construct: shRNA TRCN0000279137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023605.2-1784s21c1
DNA Barcode:
ATGGAGTTGGAGCTTACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo9 (71538)
Gene Description:
f-box protein 9
Transcript:
RefSeq NM_023605.2 (CURRENT)
Match location:
Position 1784 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAGTTGGAGCTTACTTTA
Hairpin Sequence:
5'-CCGG-ATGGAGTTGGAGCTTACTTTA-CTCGAG-TAAAGTAAGCTCCAACTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAGTTGGAGCTTACTTTACTCGAGTAAAGTAAGCTCCAACTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAGTTGGAGCTTACTTTACTCGAGTAAAGTAAGCTCCAACTCCAT-3'

Other clones with same target sequence:

(none)