Construct: shRNA TRCN0000279217

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008549.1-1687s21c1
DNA Barcode:
GCCTTGAAACAAGCTCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Man2a1 (17158)
Gene Description:
mannosidase 2, alpha 1
Transcript:
RefSeq NM_008549.1 (NON-CURRENT)
Match location:
Position 1687 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279217)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGAAACAAGCTCAGAAA
Hairpin Sequence:
5'-CCGG-GCCTTGAAACAAGCTCAGAAA-CTCGAG-TTTCTGAGCTTGTTTCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGAAACAAGCTCAGAAACTCGAGTTTCTGAGCTTGTTTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGAAACAAGCTCAGAAACTCGAGTTTCTGAGCTTGTTTCAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000018475