Construct: shRNA TRCN0000279458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008064.2-1265s21c1
DNA Barcode:
CAAGAGCGTTGTGCAACAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gaa (14387)
Gene Description:
glucosidase, alpha, acid
Transcript:
RefSeq NM_008064.2 (NON-CURRENT)
Match location:
Position 1265 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGCGTTGTGCAACAATA
Hairpin Sequence:
5'-CCGG-CAAGAGCGTTGTGCAACAATA-CTCGAG-TATTGTTGCACAACGCTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGCGTTGTGCAACAATACTCGAGTATTGTTGCACAACGCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGCGTTGTGCAACAATACTCGAGTATTGTTGCACAACGCTCTTG-3'

Other clones with same target sequence:

  1. TRCN0000190906