Construct: shRNA TRCN0000279483

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175411.4-521s21c1
DNA Barcode:
CACTGAAGTTATGAGAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam118b (109229)
Gene Description:
family with sequence similarity 118, member B
Transcript:
RefSeq NM_175411.4 (NON-CURRENT)
Match location:
Position 521 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279483)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGAAGTTATGAGAGAGAT
Hairpin Sequence:
5'-CCGG-CACTGAAGTTATGAGAGAGAT-CTCGAG-ATCTCTCTCATAACTTCAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGAAGTTATGAGAGAGATCTCGAGATCTCTCTCATAACTTCAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGAAGTTATGAGAGAGATCTCGAGATCTCTCTCATAACTTCAGTG-3'

Other clones with same target sequence:

  1. TRCN0000176645