Construct: shRNA TRCN0000279603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030650.1-1316s21c1
DNA Barcode:
CAGACAAACCAAGTGATTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LNPK (80856)
Gene Description:
lunapark, ER junction formation factor
Transcript:
RefSeq NM_030650.1 (NON-CURRENT)
Match location:
Position 1316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACAAACCAAGTGATTGAA
Hairpin Sequence:
5'-CCGG-CAGACAAACCAAGTGATTGAA-CTCGAG-TTCAATCACTTGGTTTGTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACAAACCAAGTGATTGAACTCGAGTTCAATCACTTGGTTTGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACAAACCAAGTGATTGAACTCGAGTTCAATCACTTGGTTTGTCTG-3'

Other clones with same target sequence:

  1. TRCN0000128139