Construct: shRNA TRCN0000279689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014717.1-2419s21c1
DNA Barcode:
CGGACATCCCATCACCTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF536 (9745)
Gene Description:
zinc finger protein 536
Transcript:
RefSeq NM_014717.1 (NON-CURRENT)
Match location:
Position 2419 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACATCCCATCACCTTAAG
Hairpin Sequence:
5'-CCGG-CGGACATCCCATCACCTTAAG-CTCGAG-CTTAAGGTGATGGGATGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACATCCCATCACCTTAAGCTCGAGCTTAAGGTGATGGGATGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACATCCCATCACCTTAAGCTCGAGCTTAAGGTGATGGGATGTCCG-3'

Other clones with same target sequence:

(none)