Construct: shRNA TRCN0000279762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004635.3-1291s21c1
DNA Barcode:
GCCTGCATAACAGACTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK3 (7867)
Gene Description:
MAPK activated protein kinase 3
Transcript:
RefSeq NM_004635.3 (NON-CURRENT)
Match location:
Position 1291 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGCATAACAGACTGAAAT
Hairpin Sequence:
5'-CCGG-GCCTGCATAACAGACTGAAAT-CTCGAG-ATTTCAGTCTGTTATGCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGCATAACAGACTGAAATCTCGAGATTTCAGTCTGTTATGCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGCATAACAGACTGAAATCTCGAGATTTCAGTCTGTTATGCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000006153