Construct: shRNA TRCN0000279768

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016422.3-734s21c1
DNA Barcode:
AGATGACTGGAGCAAATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF141 (50862)
Gene Description:
ring finger protein 141
Transcript:
RefSeq NM_016422.3 (NON-CURRENT)
Match location:
Position 734 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279768)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATGACTGGAGCAAATGAAT
Hairpin Sequence:
5'-CCGG-AGATGACTGGAGCAAATGAAT-CTCGAG-ATTCATTTGCTCCAGTCATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATGACTGGAGCAAATGAATCTCGAGATTCATTTGCTCCAGTCATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATGACTGGAGCAAATGAATCTCGAGATTCATTTGCTCCAGTCATCT-3'

Other clones with same target sequence:

  1. TRCN0000004181