Construct: shRNA TRCN0000279794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152649.2-916s21c1
DNA Barcode:
GAAGCTTCACTGAGACGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MLKL (197259)
Gene Description:
mixed lineage kinase domain like pseudokinase
Transcript:
RefSeq NM_152649.2 (NON-CURRENT)
Match location:
Position 916 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTTCACTGAGACGATTA
Hairpin Sequence:
5'-CCGG-GAAGCTTCACTGAGACGATTA-CTCGAG-TAATCGTCTCAGTGAAGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTTCACTGAGACGATTACTCGAGTAATCGTCTCAGTGAAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTTCACTGAGACGATTACTCGAGTAATCGTCTCAGTGAAGCTTC-3'

Other clones with same target sequence:

  1. TRCN0000196317