Construct: shRNA TRCN0000279809
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002577.4-1742s21c1
- DNA Barcode:
- CGGGATTTCTTAAATCGATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK2 (5062)
- Gene Description:
- p21 (RAC1) activated kinase 2
- Transcript:
- RefSeq NM_002577.4 (CURRENT)
- Match location:
- Position 1742 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000279809)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGGGATTTCTTAAATCGATGT
- Hairpin Sequence:
-
5'-CCGG-CGGGATTTCTTAAATCGATGT-CTCGAG-ACATCGATTTAAGAAATCCCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGGGATTTCTTAAATCGATGTCTCGAGACATCGATTTAAGAAATCCCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGGGATTTCTTAAATCGATGTCTCGAGACATCGATTTAAGAAATCCCG-3'
Other clones with same target sequence:
-
TRCN0000194671
