Construct: shRNA TRCN0000279818
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_003341.3-593s21c1
- DNA Barcode:
- GTCCAGCACTAACCATTTCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UBE2E1 (7324)
- Gene Description:
- ubiquitin conjugating enzyme E2 E1
- Transcript:
- RefSeq NM_003341.3 (NON-CURRENT)
- Match location:
- Position 593 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000279818)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTCCAGCACTAACCATTTCTA
- Hairpin Sequence:
-
5'-CCGG-GTCCAGCACTAACCATTTCTA-CTCGAG-TAGAAATGGTTAGTGCTGGAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTCCAGCACTAACCATTTCTACTCGAGTAGAAATGGTTAGTGCTGGACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTCCAGCACTAACCATTTCTACTCGAGTAGAAATGGTTAGTGCTGGAC-3'
Other clones with same target sequence:
-
TRCN0000004018
