Construct: shRNA TRCN0000279872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017582.6-1026s21c1
DNA Barcode:
CCGACTTCATTCTACTTAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2Q1 (55585)
Gene Description:
ubiquitin conjugating enzyme E2 Q1
Transcript:
RefSeq NM_017582.6 (NON-CURRENT)
Match location:
Position 1026 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000279872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTTCATTCTACTTAACT
Hairpin Sequence:
5'-CCGG-CCGACTTCATTCTACTTAACT-CTCGAG-AGTTAAGTAGAATGAAGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTTCATTCTACTTAACTCTCGAGAGTTAAGTAGAATGAAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTTCATTCTACTTAACTCTCGAGAGTTAAGTAGAATGAAGTCGG-3'

Other clones with same target sequence:

  1. TRCN0000004202