Construct: shRNA TRCN0000280022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007315.3-2907s21c1
DNA Barcode:
CCCAAAGTATCAGGACGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAT1 (6772)
Gene Description:
signal transducer and activator of transcription 1
Transcript:
RefSeq NM_007315.3 (CURRENT)
Match location:
Position 2907 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAAGTATCAGGACGAGAA
Hairpin Sequence:
5'-CCGG-CCCAAAGTATCAGGACGAGAA-CTCGAG-TTCTCGTCCTGATACTTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAAGTATCAGGACGAGAACTCGAGTTCTCGTCCTGATACTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAAGTATCAGGACGAGAACTCGAGTTCTCGTCCTGATACTTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000004268