Construct: shRNA TRCN0000280029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014791.2-1805s21c1
DNA Barcode:
GACTAAAGCTTCACTATAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MELK (9833)
Gene Description:
maternal embryonic leucine zipper kinase
Transcript:
RefSeq NM_014791.2 (NON-CURRENT)
Match location:
Position 1805 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTAAAGCTTCACTATAATG
Hairpin Sequence:
5'-CCGG-GACTAAAGCTTCACTATAATG-CTCGAG-CATTATAGTGAAGCTTTAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTAAAGCTTCACTATAATGCTCGAGCATTATAGTGAAGCTTTAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTAAAGCTTCACTATAATGCTCGAGCATTATAGTGAAGCTTTAGTC-3'

Other clones with same target sequence:

  1. TRCN0000196420