Construct: shRNA TRCN0000280046
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021003.4-1528s21c1
- DNA Barcode:
- GCCGTTTACAATAGACTGAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PPM1A (5494)
- Gene Description:
- protein phosphatase, Mg2+/Mn2+ dependent 1A
- Transcript:
- RefSeq NM_021003.4 (NON-CURRENT)
- Match location:
- Position 1528 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000280046)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCGTTTACAATAGACTGAAT
- Hairpin Sequence:
-
5'-CCGG-GCCGTTTACAATAGACTGAAT-CTCGAG-ATTCAGTCTATTGTAAACGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCGTTTACAATAGACTGAATCTCGAGATTCAGTCTATTGTAAACGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCGTTTACAATAGACTGAATCTCGAGATTCAGTCTATTGTAAACGGC-3'
Other clones with same target sequence:
-
TRCN0000002572
