Construct: shRNA TRCN0000280126

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013907.2-1160s21c1
DNA Barcode:
GCCTCATGACAGTACCTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxw4 (30838)
Gene Description:
F-box and WD-40 domain protein 4
Transcript:
RefSeq NM_013907.2 (CURRENT)
Match location:
Position 1160 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280126)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCATGACAGTACCTTCTA
Hairpin Sequence:
5'-CCGG-GCCTCATGACAGTACCTTCTA-CTCGAG-TAGAAGGTACTGTCATGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCATGACAGTACCTTCTACTCGAGTAGAAGGTACTGTCATGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCATGACAGTACCTTCTACTCGAGTAGAAGGTACTGTCATGAGGC-3'

Other clones with same target sequence:

  1. TRCN0000012820