Construct: shRNA TRCN0000280223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023813.3-1124s21c1
DNA Barcode:
GCATAGACTGTATCAGCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camk2d (108058)
Gene Description:
calcium/calmodulin-dependent protein kinase II, delta
Transcript:
RefSeq NM_023813.3 (NON-CURRENT)
Match location:
Position 1124 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAGACTGTATCAGCAGAT
Hairpin Sequence:
5'-CCGG-GCATAGACTGTATCAGCAGAT-CTCGAG-ATCTGCTGATACAGTCTATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAGACTGTATCAGCAGATCTCGAGATCTGCTGATACAGTCTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAGACTGTATCAGCAGATCTCGAGATCTGCTGATACAGTCTATGC-3'

Other clones with same target sequence:

  1. TRCN0000012481