Construct: shRNA TRCN0000280243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199141.1-2075s21c1
DNA Barcode:
CTATGGGAACTGGGACACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CARM1 (10498)
Gene Description:
coactivator associated arginine methyltransferase 1
Transcript:
RefSeq NM_199141.1 (NON-CURRENT)
Match location:
Position 2075 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGGGAACTGGGACACTTT
Hairpin Sequence:
5'-CCGG-CTATGGGAACTGGGACACTTT-CTCGAG-AAAGTGTCCCAGTTCCCATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGGGAACTGGGACACTTTCTCGAGAAAGTGTCCCAGTTCCCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGGGAACTGGGACACTTTCTCGAGAAAGTGTCCCAGTTCCCATAG-3'

Other clones with same target sequence:

  1. TRCN0000007166