Construct: shRNA TRCN0000280260

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004606.3-1480s21c1
DNA Barcode:
GCCACTCTTGATGATGACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1 (6872)
Gene Description:
TATA-box binding protein associated factor 1
Transcript:
RefSeq NM_004606.3 (NON-CURRENT)
Match location:
Position 1480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280260)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTCTTGATGATGACAAA
Hairpin Sequence:
5'-CCGG-GCCACTCTTGATGATGACAAA-CTCGAG-TTTGTCATCATCAAGAGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTCTTGATGATGACAAACTCGAGTTTGTCATCATCAAGAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTCTTGATGATGACAAACTCGAGTTTGTCATCATCAAGAGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000006288