Construct: shRNA TRCN0000280296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024041.2-430s21c1
DNA Barcode:
GCTAACTCAGACACGACTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCNM1 (79005)
Gene Description:
sodium channel modifier 1
Transcript:
RefSeq NM_024041.2 (NON-CURRENT)
Match location:
Position 430 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACTCAGACACGACTTAT
Hairpin Sequence:
5'-CCGG-GCTAACTCAGACACGACTTAT-CTCGAG-ATAAGTCGTGTCTGAGTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACTCAGACACGACTTATCTCGAGATAAGTCGTGTCTGAGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACTCAGACACGACTTATCTCGAGATAAGTCGTGTCTGAGTTAGC-3'

Other clones with same target sequence:

  1. TRCN0000005674