Construct: shRNA TRCN0000280303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201280.1-434s21c1
DNA Barcode:
CCACTTAGTAGCTAGTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BLOC1S5 (63915)
Gene Description:
biogenesis of lysosomal organelles complex 1 subunit 5
Transcript:
RefSeq NM_201280.1 (NON-CURRENT)
Match location:
Position 434 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTAGTAGCTAGTGAGAA
Hairpin Sequence:
5'-CCGG-CCACTTAGTAGCTAGTGAGAA-CTCGAG-TTCTCACTAGCTACTAAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTAGTAGCTAGTGAGAACTCGAGTTCTCACTAGCTACTAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTAGTAGCTAGTGAGAACTCGAGTTCTCACTAGCTACTAAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000128812