Construct: shRNA TRCN0000280345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004128.2-389s21c1
DNA Barcode:
GCTCCTAGAGAACATCCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2F2 (2963)
Gene Description:
general transcription factor IIF subunit 2
Transcript:
RefSeq NM_004128.2 (NON-CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCTAGAGAACATCCATTT
Hairpin Sequence:
5'-CCGG-GCTCCTAGAGAACATCCATTT-CTCGAG-AAATGGATGTTCTCTAGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCTAGAGAACATCCATTTCTCGAGAAATGGATGTTCTCTAGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCTAGAGAACATCCATTTCTCGAGAAATGGATGTTCTCTAGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000020892