Construct: shRNA TRCN0000280347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004128.2-1257s21c1
DNA Barcode:
GCTGAGGTAATACAGATCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2F2 (2963)
Gene Description:
general transcription factor IIF subunit 2
Transcript:
RefSeq NM_004128.2 (NON-CURRENT)
Match location:
Position 1257 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGGTAATACAGATCCAA
Hairpin Sequence:
5'-CCGG-GCTGAGGTAATACAGATCCAA-CTCGAG-TTGGATCTGTATTACCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGGTAATACAGATCCAACTCGAGTTGGATCTGTATTACCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGGTAATACAGATCCAACTCGAGTTGGATCTGTATTACCTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000020889