Construct: shRNA TRCN0000280421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003111.3-3726s21c1
DNA Barcode:
GCTGGTCTTGTAAGAGCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SP3 (6670)
Gene Description:
Sp3 transcription factor
Transcript:
RefSeq NM_003111.3 (NON-CURRENT)
Match location:
Position 3726 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTCTTGTAAGAGCTATA
Hairpin Sequence:
5'-CCGG-GCTGGTCTTGTAAGAGCTATA-CTCGAG-TATAGCTCTTACAAGACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTCTTGTAAGAGCTATACTCGAGTATAGCTCTTACAAGACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTCTTGTAAGAGCTATACTCGAGTATAGCTCTTACAAGACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000020489