Construct: shRNA TRCN0000280483

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008011.2-1728s21c1
DNA Barcode:
GCTAATCGGAAGACACAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fgfr4 (14186)
Gene Description:
fibroblast growth factor receptor 4
Transcript:
RefSeq NM_008011.2 (CURRENT)
Match location:
Position 1728 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280483)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAATCGGAAGACACAAGAA
Hairpin Sequence:
5'-CCGG-GCTAATCGGAAGACACAAGAA-CTCGAG-TTCTTGTGTCTTCCGATTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAATCGGAAGACACAAGAACTCGAGTTCTTGTGTCTTCCGATTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAATCGGAAGACACAAGAACTCGAGTTCTTGTGTCTTCCGATTAGC-3'

Other clones with same target sequence:

  1. TRCN0000023567