Construct: shRNA TRCN0000280527
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_019965.2-407s21c1
- DNA Barcode:
- AGCAGTGTAAAGATTACTATG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Dnajb12 (56709)
- Gene Description:
- DnaJ heat shock protein family (Hsp40) member B12
- Transcript:
- RefSeq NM_019965.2 (CURRENT)
- Match location:
- Position 407 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000280527)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGCAGTGTAAAGATTACTATG
- Hairpin Sequence:
-
5'-CCGG-AGCAGTGTAAAGATTACTATG-CTCGAG-CATAGTAATCTTTACACTGCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGCAGTGTAAAGATTACTATGCTCGAGCATAGTAATCTTTACACTGCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGCAGTGTAAAGATTACTATGCTCGAGCATAGTAATCTTTACACTGCT-3'
Other clones with same target sequence:
(none)
