Construct: shRNA TRCN0000280553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021462.3-486s21c1
DNA Barcode:
CCTGGACTTCCTGCATAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mknk2 (17347)
Gene Description:
MAP kinase-interacting serine/threonine kinase 2
Transcript:
RefSeq NM_021462.3 (NON-CURRENT)
Match location:
Position 486 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGACTTCCTGCATAACAA
Hairpin Sequence:
5'-CCGG-CCTGGACTTCCTGCATAACAA-CTCGAG-TTGTTATGCAGGAAGTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGACTTCCTGCATAACAACTCGAGTTGTTATGCAGGAAGTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGACTTCCTGCATAACAACTCGAGTTGTTATGCAGGAAGTCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000221178