Construct: shRNA TRCN0000280555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011062.4-1608s21c1
DNA Barcode:
CCTGTCAACAAGGTCTTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdpk1 (18607)
Gene Description:
3-phosphoinositide dependent protein kinase 1
Transcript:
RefSeq NM_011062.4 (CURRENT)
Match location:
Position 1608 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCAACAAGGTCTTGAAA
Hairpin Sequence:
5'-CCGG-CCTGTCAACAAGGTCTTGAAA-CTCGAG-TTTCAAGACCTTGTTGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCAACAAGGTCTTGAAACTCGAGTTTCAAGACCTTGTTGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCAACAAGGTCTTGAAACTCGAGTTTCAAGACCTTGTTGACAGG-3'

Other clones with same target sequence:

  1. TRCN0000022735