Construct: shRNA TRCN0000280589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004339.2-2322s21c1
DNA Barcode:
CCCAGTTCTTCAGGCTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTTG1IP (754)
Gene Description:
PTTG1 interacting protein
Transcript:
RefSeq NM_004339.2 (NON-CURRENT)
Match location:
Position 2322 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTTCTTCAGGCTGATTT
Hairpin Sequence:
5'-CCGG-CCCAGTTCTTCAGGCTGATTT-CTCGAG-AAATCAGCCTGAAGAACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTTCTTCAGGCTGATTTCTCGAGAAATCAGCCTGAAGAACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTTCTTCAGGCTGATTTCTCGAGAAATCAGCCTGAAGAACTGGG-3'

Other clones with same target sequence:

  1. TRCN0000166637