Construct: shRNA TRCN0000280706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020179.2-342s21c1
DNA Barcode:
GCAGATCACTACAGTGGTGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMCO4 (56935)
Gene Description:
single-pass membrane protein with coiled-coil domains 4
Transcript:
RefSeq NM_020179.2 (NON-CURRENT)
Match location:
Position 342 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATCACTACAGTGGTGCT
Hairpin Sequence:
5'-CCGG-GCAGATCACTACAGTGGTGCT-CTCGAG-AGCACCACTGTAGTGATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATCACTACAGTGGTGCTCTCGAGAGCACCACTGTAGTGATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATCACTACAGTGGTGCTCTCGAGAGCACCACTGTAGTGATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000122633