Construct: shRNA TRCN0000280773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020300.3-694s21c1
DNA Barcode:
GCATTGCCAATATCCTGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MGST1 (4257)
Gene Description:
microsomal glutathione S-transferase 1
Transcript:
RefSeq NM_020300.3 (NON-CURRENT)
Match location:
Position 694 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGCCAATATCCTGTATT
Hairpin Sequence:
5'-CCGG-GCATTGCCAATATCCTGTATT-CTCGAG-AATACAGGATATTGGCAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGCCAATATCCTGTATTCTCGAGAATACAGGATATTGGCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGCCAATATCCTGTATTCTCGAGAATACAGGATATTGGCAATGC-3'

Other clones with same target sequence:

  1. TRCN0000158997