Construct: shRNA TRCN0000280892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024078.1-1202s21c1
DNA Barcode:
CCTGCCTTTCATCTGTAACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOC4L (79050)
Gene Description:
nucleolar complex associated 4 homolog
Transcript:
RefSeq NM_024078.1 (NON-CURRENT)
Match location:
Position 1202 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCTTTCATCTGTAACCT
Hairpin Sequence:
5'-CCGG-CCTGCCTTTCATCTGTAACCT-CTCGAG-AGGTTACAGATGAAAGGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCTTTCATCTGTAACCTCTCGAGAGGTTACAGATGAAAGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCTTTCATCTGTAACCTCTCGAGAGGTTACAGATGAAAGGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000122580