Construct: shRNA TRCN0000280977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021824.3-759s21c1
DNA Barcode:
CGTTAACTACACCCAAGACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NIF3L1 (60491)
Gene Description:
NGG1 interacting factor 3 like 1
Transcript:
RefSeq NM_021824.3 (CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTAACTACACCCAAGACCT
Hairpin Sequence:
5'-CCGG-CGTTAACTACACCCAAGACCT-CTCGAG-AGGTCTTGGGTGTAGTTAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTAACTACACCCAAGACCTCTCGAGAGGTCTTGGGTGTAGTTAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTAACTACACCCAAGACCTCTCGAGAGGTCTTGGGTGTAGTTAACG-3'

Other clones with same target sequence:

  1. TRCN0000129337