Construct: shRNA TRCN0000280983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000284.2-214s21c1
DNA Barcode:
GCTGGTAGCATCCCGTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDHA1 (5160)
Gene Description:
pyruvate dehydrogenase E1 alpha 1 subunit
Transcript:
RefSeq NM_000284.2 (NON-CURRENT)
Match location:
Position 214 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000280983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTAGCATCCCGTAATTT
Hairpin Sequence:
5'-CCGG-GCTGGTAGCATCCCGTAATTT-CTCGAG-AAATTACGGGATGCTACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTAGCATCCCGTAATTTCTCGAGAAATTACGGGATGCTACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTAGCATCCCGTAATTTCTCGAGAAATTACGGGATGCTACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000220889