Construct: shRNA TRCN0000281022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017432.3-491s21c1
DNA Barcode:
CTACTCTGACTCCACTGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTOV1 (53635)
Gene Description:
PTOV1 extended AT-hook containing adaptor protein
Transcript:
RefSeq NM_017432.3 (NON-CURRENT)
Match location:
Position 491 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTCTGACTCCACTGCAAA
Hairpin Sequence:
5'-CCGG-CTACTCTGACTCCACTGCAAA-CTCGAG-TTTGCAGTGGAGTCAGAGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTCTGACTCCACTGCAAACTCGAGTTTGCAGTGGAGTCAGAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTCTGACTCCACTGCAAACTCGAGTTTGCAGTGGAGTCAGAGTAG-3'

Other clones with same target sequence:

  1. TRCN0000140055