Construct: shRNA TRCN0000281090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017432.3-927s21c1
DNA Barcode:
GTCAACAACAAGTTTCTGGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTOV1 (53635)
Gene Description:
PTOV1 extended AT-hook containing adaptor protein
Transcript:
RefSeq NM_017432.3 (NON-CURRENT)
Match location:
Position 927 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAACAACAAGTTTCTGGCA
Hairpin Sequence:
5'-CCGG-GTCAACAACAAGTTTCTGGCA-CTCGAG-TGCCAGAAACTTGTTGTTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAACAACAAGTTTCTGGCACTCGAGTGCCAGAAACTTGTTGTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAACAACAAGTTTCTGGCACTCGAGTGCCAGAAACTTGTTGTTGAC-3'

Other clones with same target sequence:

  1. TRCN0000143781