Construct: shRNA TRCN0000281125

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014744.2-3655s21c1
DNA Barcode:
GAGAACGAACAGATCACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D5 (9779)
Gene Description:
TBC1 domain family member 5
Transcript:
RefSeq NM_014744.2 (CURRENT)
Match location:
Position 3655 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281125)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAACGAACAGATCACCATT
Hairpin Sequence:
5'-CCGG-GAGAACGAACAGATCACCATT-CTCGAG-AATGGTGATCTGTTCGTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAACGAACAGATCACCATTCTCGAGAATGGTGATCTGTTCGTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAACGAACAGATCACCATTCTCGAGAATGGTGATCTGTTCGTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000136647