Construct: shRNA TRCN0000281131

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006662.2-1504s21c1
DNA Barcode:
CCCTCAGGTGTTGGAGATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRCAP (10847)
Gene Description:
Snf2 related CREBBP activator protein
Transcript:
RefSeq NM_006662.2 (NON-CURRENT)
Match location:
Position 1504 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281131)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGGTGTTGGAGATAAA
Hairpin Sequence:
5'-CCGG-CCCTCAGGTGTTGGAGATAAA-CTCGAG-TTTATCTCCAACACCTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGGTGTTGGAGATAAACTCGAGTTTATCTCCAACACCTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGGTGTTGGAGATAAACTCGAGTTTATCTCCAACACCTGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000021357