Construct: shRNA TRCN0000281135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015315.3-2694s21c1
DNA Barcode:
GCTGGACATATTCAAGGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LARP1 (23367)
Gene Description:
La ribonucleoprotein 1, translational regulator
Transcript:
RefSeq NM_015315.3 (NON-CURRENT)
Match location:
Position 2694 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGACATATTCAAGGATTT
Hairpin Sequence:
5'-CCGG-GCTGGACATATTCAAGGATTT-CTCGAG-AAATCCTTGAATATGTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGACATATTCAAGGATTTCTCGAGAAATCCTTGAATATGTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGACATATTCAAGGATTTCTCGAGAAATCCTTGAATATGTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000150575