Construct: shRNA TRCN0000281142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005791.1-725s21c1
DNA Barcode:
GATGACAAGGAGGACCTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPHOSPH10 (10199)
Gene Description:
M-phase phosphoprotein 10
Transcript:
RefSeq NM_005791.1 (NON-CURRENT)
Match location:
Position 725 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGACAAGGAGGACCTAGAA
Hairpin Sequence:
5'-CCGG-GATGACAAGGAGGACCTAGAA-CTCGAG-TTCTAGGTCCTCCTTGTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGACAAGGAGGACCTAGAACTCGAGTTCTAGGTCCTCCTTGTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGACAAGGAGGACCTAGAACTCGAGTTCTAGGTCCTCCTTGTCATC-3'

Other clones with same target sequence:

  1. TRCN0000163143