Construct: shRNA TRCN0000281173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144963.2-2115s21c1
DNA Barcode:
GCTCAGATGTAAATGGGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM91A1 (157769)
Gene Description:
family with sequence similarity 91 member A1
Transcript:
RefSeq NM_144963.2 (NON-CURRENT)
Match location:
Position 2115 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGATGTAAATGGGAGTA
Hairpin Sequence:
5'-CCGG-GCTCAGATGTAAATGGGAGTA-CTCGAG-TACTCCCATTTACATCTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGATGTAAATGGGAGTACTCGAGTACTCCCATTTACATCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGATGTAAATGGGAGTACTCGAGTACTCCCATTTACATCTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000161570