Construct: shRNA TRCN0000281199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006662.2-6499s21c1
DNA Barcode:
CCAGTATGATTGCGGAAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRCAP (10847)
Gene Description:
Snf2 related CREBBP activator protein
Transcript:
RefSeq NM_006662.2 (NON-CURRENT)
Match location:
Position 6499 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTATGATTGCGGAAAGTT
Hairpin Sequence:
5'-CCGG-CCAGTATGATTGCGGAAAGTT-CTCGAG-AACTTTCCGCAATCATACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTATGATTGCGGAAAGTTCTCGAGAACTTTCCGCAATCATACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTATGATTGCGGAAAGTTCTCGAGAACTTTCCGCAATCATACTGG-3'

Other clones with same target sequence:

  1. TRCN0000021355