Construct: shRNA TRCN0000281229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020841.4-1538s21c1
DNA Barcode:
GTGACACAGATACATCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OSBPL8 (114882)
Gene Description:
oxysterol binding protein like 8
Transcript:
RefSeq NM_020841.4 (NON-CURRENT)
Match location:
Position 1538 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACACAGATACATCAGAAA
Hairpin Sequence:
5'-CCGG-GTGACACAGATACATCAGAAA-CTCGAG-TTTCTGATGTATCTGTGTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACACAGATACATCAGAAACTCGAGTTTCTGATGTATCTGTGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACACAGATACATCAGAAACTCGAGTTTCTGATGTATCTGTGTCAC-3'

Other clones with same target sequence:

  1. TRCN0000147289