Construct: shRNA TRCN0000281239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144963.2-1223s21c1
DNA Barcode:
GCCTTCTTAATGATGGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM91A1 (157769)
Gene Description:
family with sequence similarity 91 member A1
Transcript:
RefSeq NM_144963.2 (NON-CURRENT)
Match location:
Position 1223 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTTAATGATGGGAAAT
Hairpin Sequence:
5'-CCGG-GCCTTCTTAATGATGGGAAAT-CTCGAG-ATTTCCCATCATTAAGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTTAATGATGGGAAATCTCGAGATTTCCCATCATTAAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTTAATGATGGGAAATCTCGAGATTTCCCATCATTAAGAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000159145