Construct: shRNA TRCN0000281253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006317.3-225s21c1
DNA Barcode:
GACGAGAAAGCCAAGGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BASP1 (10409)
Gene Description:
brain abundant membrane attached signal protein 1
Transcript:
RefSeq NM_006317.3 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGAGAAAGCCAAGGAGAAA
Hairpin Sequence:
5'-CCGG-GACGAGAAAGCCAAGGAGAAA-CTCGAG-TTTCTCCTTGGCTTTCTCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGAGAAAGCCAAGGAGAAACTCGAGTTTCTCCTTGGCTTTCTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGAGAAAGCCAAGGAGAAACTCGAGTTTCTCCTTGGCTTTCTCGTC-3'

Other clones with same target sequence:

  1. TRCN0000180477