Construct: shRNA TRCN0000281415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058197.3-904s21c1
DNA Barcode:
AGTAACCATGCCCGCATAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN2A (1029)
Gene Description:
cyclin dependent kinase inhibitor 2A
Transcript:
RefSeq NM_058197.3 (NON-CURRENT)
Match location:
Position 904 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAACCATGCCCGCATAGAT
Hairpin Sequence:
5'-CCGG-AGTAACCATGCCCGCATAGAT-CTCGAG-ATCTATGCGGGCATGGTTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAACCATGCCCGCATAGATCTCGAGATCTATGCGGGCATGGTTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAACCATGCCCGCATAGATCTCGAGATCTATGCGGGCATGGTTACT-3'

Other clones with same target sequence:

(none)