Construct: shRNA TRCN0000281426

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010441.2-1179s21c1
DNA Barcode:
GAGTCCGCAGAGGAGGATTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmga2 (15364)
Gene Description:
high mobility group AT-hook 2
Transcript:
RefSeq NM_010441.2 (NON-CURRENT)
Match location:
Position 1179 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281426)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCCGCAGAGGAGGATTAG
Hairpin Sequence:
5'-CCGG-GAGTCCGCAGAGGAGGATTAG-CTCGAG-CTAATCCTCCTCTGCGGACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCCGCAGAGGAGGATTAGCTCGAGCTAATCCTCCTCTGCGGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCCGCAGAGGAGGATTAGCTCGAGCTAATCCTCCTCTGCGGACTC-3'

Other clones with same target sequence:

(none)