Construct: shRNA TRCN0000281542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098409.1-149s21c1
DNA Barcode:
GCCTAGACCAAGGCGCTATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE12G (645073)
Gene Description:
G antigen 12G
Transcript:
RefSeq NM_001098409.1 (NON-CURRENT)
Match location:
Position 149 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGACCAAGGCGCTATGT
Hairpin Sequence:
5'-CCGG-GCCTAGACCAAGGCGCTATGT-CTCGAG-ACATAGCGCCTTGGTCTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGACCAAGGCGCTATGTCTCGAGACATAGCGCCTTGGTCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGACCAAGGCGCTATGTCTCGAGACATAGCGCCTTGGTCTAGGC-3'

Other clones with same target sequence:

(none)