Construct: shRNA TRCN0000281602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001100418.1-664s21c1
DNA Barcode:
ATGGAGGCGATCAGCGAGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
REX1BD (55049)
Gene Description:
required for excision 1-B domain containing
Transcript:
RefSeq NM_001100418.1 (CURRENT)
Match location:
Position 664 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAGGCGATCAGCGAGGTT
Hairpin Sequence:
5'-CCGG-ATGGAGGCGATCAGCGAGGTT-CTCGAG-AACCTCGCTGATCGCCTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAGGCGATCAGCGAGGTTCTCGAGAACCTCGCTGATCGCCTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAGGCGATCAGCGAGGTTCTCGAGAACCTCGCTGATCGCCTCCAT-3'

Other clones with same target sequence:

(none)